Recombinant human CXCL8 is expressed in E. coli, refolded and purified to yield the mature form of protein that is secreted from cells after cleavage of the signal peptide. Human CXCL8 is a 77 amino acid protein that is the prototypical CXC chemokine which is expressed by fibroblasts and keratinocytes. Also known as Interleukin 8, this chemokine binds and activates two G protein-coupled receptors, CXCR1/CXCR2 on a variety of cells such as neutrophils, T-cells, endothelial cells, macrophages and keratinocytes. CXCL8 is N-terminally processed to yield the major in vivo species, the 6-77 variant. (link to CXCL8 6-77 product page)
Amino Acid Sequence:
|Molecular Weight:||8381.77 Daltons|
|Purity:||Assessed by HPLC, Mass Spectrometry, and NMR|
|Source:||Human protein expressed in E. coli|
|Physical Form:||Lyophilized powder|
|Solubility:||Freely Soluble in Water|
|Storage Conditions:||Store at -20o C|
Additional comments or descriptive information:
Clore, G. M., Appella, E., Yamada, M., Matsushima, K., & Gronenborn, A. M. (1989). Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopy.
J Biol Chem, 264(32), 18907-18911. Clark-Lewis, I., Schumacher, C., Baggiolini, M., & Moser, B. (1991). Structure-activity relationships of interleukin-8 determined using chemically synthesized analogs. Critical role of NH2-terminal residues and evidence for uncoupling of neutrophil chemotaxis, exocytosis, and receptor binding activities. J Biol Chem, 266(34), 23128-23134.
Larsen, C. G., Anderson, A. O., Oppenheim, J. J., & Matsushima, K. (1989). Production of interleukin-8 by human dermal fibroblasts and keratinocytes in response to interleukin-1 or tumour necrosis factor. Immunology, 68(1), 31-36.
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Recombinant Human CXCL8
- Product Code: PFP036
- Availability: In Stock