XCL2 protein is expressed in E. coli, processed, refolded and purified to yield the native, secreted form of the mature chemokine.
XCL2 is a ligand for the G protein-coupled receptor XCR1 with and EC50 ~ 50 nM. XCL2, along with the closely related paralog XCL1, are the only two members the C-chemokine subfamily (1). Like XCL1, XCL2 shares similar structural and agonist properties. It is able to interconvert between two distinct conformations under physiological temperature and NaCl conditions. In one conformation, XCL2 exists as a monomer and is able to bind to its receptor. In the other conformation, XCL2 exists as a dimer and loses affinity for XCR1 (2,3).
Amino Acid Sequence:
|Molecular Weight:||10319.8 Daltons|
|Purity:||Assessed by HPLC, Mass Spectrometry, and NMR|
|Source:||Human protein expressed in E. coli|
|Physical Form:||Lyophilized powder|
|Solubility:||Freely Soluble in Water|
|Storage Conditions:||Store at -20o C|
Additional comments or descriptive information:
(1) Yoshida, T., Imai, T., Takagi, S., Nishimura, M., Ishikawa, I., Yaoi, T., & Yoshie, O. Structure and expression of two highly related genes encoding SCM-1/human lymphotactin. FEBS Letters 395, 82-88 (1996).
(2)Tunistra, R.L., Peterson, F.C., Elgin, E.S., Pelzek, A.J., & Volkman, B.F. An Engineered Second Disulfide Bond Restricts Lymphotactin/XCL1 to a Chemokine-‐like Conformation with XCR1 Agonist Activity. Biochemistry 46, 2564-2573 (2007).
(3) Tunistra, R.L., Peterson, F.C., Kutlesa, S., Elgin, E.S., Kron, M.A., & Volkman, B.F. Interconversion between two unrelated protein folds in the lymphotactin native state. PNAS 105, 5057-5062 (2008).
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Recombinant Human XCL2 /Lymphotactin (Ltn)
- Product Code: PFP006
- Availability: In Stock