XCL1 protein is expressed in E. coli, processed, refolded and purified to yield the native, secreted form of the mature chemokine.
XCL1 is a ligand for the G protein coupled receptor XCR1 with and EC50 ~ 50 nM. It has been shown the WT XCL1 is able to interconvert between two distinct conformations under physiological conditions. In one conformation, XCL1 exists as a monomer and is able to bind to its receptor. In the other conformation, XCL1 exists as a dimer and loses affinity for XCR1 (1,2).
Amino Acid Sequence:
VGSEVSDKRT CVSLTTQRLP VSRIKTYTIT EGSLRAVIFI TKRGLKVCAD PQATWVRDVV RSMDRKSNTR NNMIQTKPTG TQQSTNTAVT LTG
|Molecular Weight:||10269.7 Daltons|
|Purity:||Assessed by HPLC, Mass Spectrometry, and NMR|
|Source:||Human protein expressed in E. coli|
|Physical Form:||Lyophilized powder|
|Solubility:||Freely Soluble in Water|
|Storage Conditions:||Store at -20o C|
Additional comments or descriptive information:
- Tunistra, R.L., Peterson, F.C., Elgin, E.S., Pelzek, A.J., & Volkman, B.F. An Engineered Second Disulfide Bond Restricts Lymphotactin/XCL1 to a Chemokine-‐like Conformation with XCR1 Agonist Activity. Biochemistry 46, 2564-‐2573 (2007).
- Tunistra, R.L., Peterson, F.C., Kutlesa, S., Elgin, E.S., Kron, M.A., & Volkman, B.F. Interconversion between two unrelated protein folds in the lymphotactin native state. PNAS 105, 5057-‐5062 (2008).
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Recombinant Human XCL1 /Lymphotactin (Ltn)
- Product Code: PFP005
- Availability: In Stock